The Technologist in Molecular Biology MB(ASCP) certification examination offered by the ASCP Board of Certification (BOC) is composed of 100 questions given in a 2 hour 30 minute time frame. All exam questions are multiple-choice with one best answer. The certification exam is administered using the format of computer adaptive testing (CAT).

The MB exam questions encompass the following content areas within Molecular Biology: Molecular Science, Molecular

Techniques, Laboratory Operations, and Applications of Molecular Testing. Each of these content areas comprises a

specific percentage of the overall 100-question exam.

Introduction to the course

Provides information on

• Brief introduction on Tutor

• Benefits from the course

• Topics/Structure of the course

• Who can enroll to the course

  
  

UNIT 1 : Replication

• Central Dogma of biology

• Replication of DNA

• Semiconservative replication

• Complimentary base pairing

• Supercoil and DNA topoisomerase

• Replication fork

• DNA synthesis catalysed by DNA Polymerase

• DNA Damage and Repair

• Nucleotide Excision Repair

• Base Excision Repair

• Mismatch Repair system

  
  

UNIT 2: Transcription

• Central Dogma of Life

• what is Transcription

• Mechanism Of Transcription

• In Prokaryotes

  • Initiation

  • elongation

  • Termination

• In Eukaryotes

  • Initiation is initiated with help of promotor region

  • TAAT Box and Pribnow Box

  • Elongation

  • Enhancers and Silencers

  • Termination

• Post Transcriptional Modification

  • Addition of Cap and Poly A tail

  • Splicing Of mRNA

  • mRNA Editing

  • Inhibitors of Transcription

  • Reverse Transcriptase and RNA Degradation.

UNIT 3 : Translation

• Genetic Code

• Features Of Genetic Code

• Codon - Anticodon Pairing

• wobble Hypothesis

• Link Between Amino Acid And Nucleic Acid

  Translation In Prokaryotes

• Initiation

• Elongation

• Termination

• How much Energy is consumed during the Process

  Translation in Eukaryotes

• Initiation with Pre Initiation complex

• Association of Ribosomal Subunit

• Elongation

• Termination using Termination Codons

• Release Factors

  Post Translational Modification

• Trimming

• Structural Reorganisation

• Modification at N and C Terminal Ends

• Covalent Modification

• How much Of energy is consumed during the process of Translation

  
  

  Unit 4: Regulation of Gene Expression

• Enhancers and silencers

• House keeping and invisible genes

• One cistron -one subunit concept

• DNA organisation and Gene expression

• Covalent Modification of Histones control Gene expression

• Regulation of Gene Expression in Prokaryotes :

• Lac operon

• structure of Lac Operon

• Tryptophan regulation in Eukaryotes

• Structure of Tryptophan Operon with structural gene

Who this course is for:

• Students who are in graduation level in the field of biology, molecular biology and Biotechnology